PPT-DNA Transfection into Prokaryotic and Eukaryotic Cells

Lily Chan and Tim Johnstone Transfection to transfer DNA into cells either eukaryotic or prokaryotic not through use of a viral vector The approaches for eukaryotic and prokaryoticcell transfection are slightly . TSW – Recognize that the presence of a nucleus determines whether a cell is prokaryotic or eukaryotic. 6.12B. Cells. There are two . basic types . of cells. Prokaryotes. or prokaryotic cells . Eukaryotes. Lily Chan and Tim Johnstone. Transfection:. . to transfer DNA into cells (either eukaryotic or prokaryotic) not through use of a viral vector. The approaches for eukaryotic and prokaryotic-cell . transfection are slightly . AJ Scielzo. Prokaryotic Flagella . Long, threadlike structures protruding from the outside surface of the cell . Main function is movement. Prokaryotic flagella are protein fibers that extend from the cell. Both are chemically similar in that they contain nucleic acids, proteins, lipids, and carbohydrates. They use the same sort of chemical reactions to . metabolise. food, build proteins and store energy. Eukaryotes, Prokaryotes and Measuring Cells. Lesson Objectives. To understand the difference between eukaryotic and prokaryotic cells.. To describe the features of a prokaryotic cell such as a bacterium.. TSW – Recognize that the presence of a nucleus determines whether a cell is prokaryotic or eukaryotic. 6.12B. Cells. There are two . basic types . of cells. Prokaryotes. or prokaryotic cells . Eukaryotes. Section 3.1. 1. Vocab and Main Concept. Cell Theory (p. 71) – Very important concept!. Know all parts. Cytoplasm (p. . 72). Organelle (p. 72). Prokaryotic cell (p. 72). Eukaryotic cell (p. 72). 2. A . Two Basic Types. Remember….cells are the basic unit of life for ALL living things.. There are two basic types of cells:. Prokaryotic cells . – found in bacteria. Eukaryotic cells . – found in . Multicellular. . Organisms. Overview. Review of key structural and genetic features of eukaryotic cells.. Remind yourself of these by your own reading. .. The origin of cells with eukaryotic organization, some 2.5 . NATE™ Catalog code: lyec-nate https://www.invivogen.com/ nate For research use only Version 19D29-ED Transient and stable transfection with NATE™ METHODS Below (and on the next page) are th Prokaryotic . Cell. The . term prokaryote is derived from the Greek word- . “prokaryote”. meaning before nuclei. . Prokaryotic . cells are simpler and smaller than the eukaryotic cells. . These cells lack membrane bound organelles. . Reagent. (. Embryonic. . Fibroblast. . Cells. , CL-173). Altogen Biosystems offers the . 3T3-L1 Transfection . Reagent among a host of 100+ cell line specific . In Vitro . Transfection Kits.. 3T3-L1 Transfection . 4. Bacteria that divide in multiple planes and form grapelike clusters are called. streptococci.. staphylococci.. coccobacilli.. vibrios.. Bacteria that divide in multiple planes and form grapelike clusters are called.  by bacterial cells. .. Transduction. occurs when foreign DNA or RNA is introduced into bacterial or eukaryotic cells . via a virus or viral vector. .. During . conjugation. , genetic material is transferred from a donor bacterium to a recipient bacterium through .