PPT-DNA Transfection into Prokaryotic and Eukaryotic Cells

Lily Chan and Tim Johnstone Transfection to transfer DNA into cells either eukaryotic or prokaryotic not through use of a viral vector The approaches for eukaryotic and prokaryoticcell transfection are slightly . AJ Scielzo. Prokaryotic Flagella . Long, threadlike structures protruding from the outside surface of the cell . Main function is movement. Prokaryotic flagella are protein fibers that extend from the cell. 2.2.1, 2.2.2: Ultrastructure of . E.coli. Mesosome. Plasmid. 2.2.3: Electron micrograph of . E.coli. Nucleoid region. cytoplasm. IB Question: . Draw and label a generalized prokaryotic cell as seen under the electron microscope. . Eukaryotes, Prokaryotes and Measuring Cells. Lesson Objectives. To understand the difference between eukaryotic and prokaryotic cells.. To describe the features of a prokaryotic cell such as a bacterium.. PROKARYOTES. EUKARYOTES. CYTOPLASM. Cell Membrane. DNA. Always single-celled. No Nucleus. DNA is a simple,. Single loop. Small Cells. (1 – 10 . um. ). Bacteria. . Can be single- or multicellular . TSW – Recognize that the presence of a nucleus determines whether a cell is prokaryotic or eukaryotic. 6.12B. Cells. There are two . basic types . of cells. Prokaryotes. or prokaryotic cells . Eukaryotes. Endosymbiosis. h. eterotrophic eukaryote. Examine these diagrams showing . how an ancestor to eukaryotic cells may have engulfed smaller heterotrophic prokaryotic cells that began producing ATP for it. Section 3.1. 1. Vocab and Main Concept. Cell Theory (p. 71) – Very important concept!. Know all parts. Cytoplasm (p. . 72). Organelle (p. 72). Prokaryotic cell (p. 72). Eukaryotic cell (p. 72). 2. A . Lily Chan and Tim Johnstone. Transfection:. . to transfer DNA into cells (either eukaryotic or prokaryotic) not through use of a viral vector. The approaches for eukaryotic and prokaryotic-cell . transfection are slightly . Multicellular. . Organisms. Overview. Review of key structural and genetic features of eukaryotic cells.. Remind yourself of these by your own reading. .. The origin of cells with eukaryotic organization, some 2.5 . Multicellular. . Organisms. Overview. Review of key structural and genetic features of eukaryotic cells.. Remind yourself of these by your own reading. .. The origin of cells with eukaryotic organization, some 2.5 . Prokaryotic . Cell. The . term prokaryote is derived from the Greek word- . “prokaryote”. meaning before nuclei. . Prokaryotic . cells are simpler and smaller than the eukaryotic cells. . These cells lack membrane bound organelles. . Eukaryotic translation. Eukaryotic mRNAs are characterized by two post-transcriptional modifications: the 5-terminal 7methyl-GTP cap and the 3-terminal poly(A) tail .. The 7methyl-GTP cap is essential for mRNA binding by eukaryotic . Reagent. (. Embryonic. . Fibroblast. . Cells. , CL-173). Altogen Biosystems offers the . 3T3-L1 Transfection . Reagent among a host of 100+ cell line specific . In Vitro . Transfection Kits.. 3T3-L1 Transfection . 4. Bacteria that divide in multiple planes and form grapelike clusters are called. streptococci.. staphylococci.. coccobacilli.. vibrios.. Bacteria that divide in multiple planes and form grapelike clusters are called.