PPT-DNA Transfection into Prokaryotic and Eukaryotic Cells

Lily Chan and Tim Johnstone Transfection to transfer DNA into cells either eukaryotic or prokaryotic not through use of a viral vector The approaches for eukaryotic and prokaryoticcell transfection are slightly . TSW – Recognize that the presence of a nucleus determines whether a cell is prokaryotic or eukaryotic. 6.12B. Cells. There are two . basic types . of cells. Prokaryotes. or prokaryotic cells . Eukaryotes. AJ Scielzo. Prokaryotic Flagella . Long, threadlike structures protruding from the outside surface of the cell . Main function is movement. Prokaryotic flagella are protein fibers that extend from the cell. Both are chemically similar in that they contain nucleic acids, proteins, lipids, and carbohydrates. They use the same sort of chemical reactions to . metabolise. food, build proteins and store energy. PROKARYOTES. EUKARYOTES. CYTOPLASM. Cell Membrane. DNA. Always single-celled. No Nucleus. DNA is a simple,. Single loop. Small Cells. (1 – 10 . um. ). Bacteria. . Can be single- or multicellular . TSW – Recognize that the presence of a nucleus determines whether a cell is prokaryotic or eukaryotic. 6.12B. Cells. There are two . basic types . of cells. Prokaryotes. or prokaryotic cells . Eukaryotes. Endosymbiosis. h. eterotrophic eukaryote. Examine these diagrams showing . how an ancestor to eukaryotic cells may have engulfed smaller heterotrophic prokaryotic cells that began producing ATP for it. Two Basic Types. Remember….cells are the basic unit of life for ALL living things.. There are two basic types of cells:. Prokaryotic cells . – found in bacteria. Eukaryotic cells . – found in . Multicellular. . Organisms. Overview. Review of key structural and genetic features of eukaryotic cells.. Remind yourself of these by your own reading. .. The origin of cells with eukaryotic organization, some 2.5 . Multicellular. . Organisms. Overview. Review of key structural and genetic features of eukaryotic cells.. Remind yourself of these by your own reading. .. The origin of cells with eukaryotic organization, some 2.5 . NATE™ Catalog code: lyec-nate https://www.invivogen.com/ nate For research use only Version 19D29-ED Transient and stable transfection with NATE™ METHODS Below (and on the next page) are th P & E UKARYOTIC C ELLS  As you have already learned, everything is made up of living cells.  And the cells themselves are made up of many different parts .  There are many different cell Science. Learning intention. To know and understand the difference between eukaryotic and prokaryotic cells.. You will need. •  A pen and paper.. •  A digital device. . Lesson 12: challenge answer. An Introduction. What is Gene Transfection?. Generation of genetically modified animals. Genetic modification of germ cells. Breeding modified individuals. To combine gene cutting or expression tools. A single guiding RNA (sgRNA; . a fusion of . tracrRNA. and crRNA. ) . binds CAS9 protein at one end, the other end guides CAS9 to target DNA sequence based on complementarity. . Gene for the CAS9 protein, encodes endonuclease, cuts both strands of the target sequence.