PPT-Blunt-end ligation Ends do not form base-pairing hydrogen bonds

ends are not close for long inefficient the rate of ligation is much lower than sticky end ligation nnnnnnnnnnnnGAT TGAnnnnnnnnnnnnn

ligation blunt amplicon plasmid blunt ligation plasmid amplicon white ends plasmidecorvendonucleaseplasmid ligated rate plasmidligation ampliconinto fragment generate dna polymerase

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