PPT-Discovery of a new type of bacterial enzyme able to cleave bonds in lignin

Kontur W S et al A heterodimeric glutathione S transferase that stereospecifically breaks lignins βRaryl ether bond reveals the diversity of bacterial β etherases . Lignin Isolation and. Acid Hydrolysis. Lignin. Large macromolecule formed by various types of substructures. Lignin. In nature is very resistant to degradation.. It is bonded in complex and various types of carbohydrates.. from plant biomass. Michele Morgante, Università di Udine. Lynd. . et. al. . Nat. . . Biotechnology. , 2008. Lynd. . et. al. . Nat. . . Biotechnology. , 2008. Costs. of . fermentable. . carbohydrate. Functionalisation. of . Lignins. Paola . Giannì. , . Heiko. Lange, Claudia . Crestini. Polyphenol. . Chemistry & Material Science Laboratory. www.polyphenols.weebly.com. Department of Chemical Sciences and Technologies. Topic 2: . Enzyme. . Kinetics. What is an Enzyme?.  . Enzymes: .  are biological molecules (proteins) that act as . catalysts*.  and help complex reactions . take place. Basically, they . break a protein down into its amino . 4746 NO. 35 / 1-2017 TECHNOLOGIES ech Talkfor Lignin Recovery and sulfuric The most quanti able bene ts to a kraft mill for installing a Lignin Recovery system are the ability to debottlene In this experiment, we will continue to study acid phosphatase kinetics.. Objective:. To establish the relationship between enzyme concentration and the rate of an enzyme catalyzed reaction.. The reaction rate will increase as the concentration of enzymes is increased but there must be a . Therapy of enzyme defects: general considerations. How many organs are affected by the enzyme defect: One organ, a few, or all organs?. How severe is the defect?. Can the defect be adequately controlled by conventional treatment?. Citation. Objective. . Develop a fast, accurate method to quantify lignin content in biomass using less energy, less starting material, and fewer toxic reagents than current processes. Approach . Completely dissolve woody biomass in 0.1 g/mL cysteine-sulfuric acid solution at 24 °C after stirring for one hour. Oates, N.C., . Abood. , A., . Schirmacher. , A.M., Alessi, A.M., Bird, S.M., Bennett, J.P., Leadbeater, D.R., Li, Y., . Dowle. , A.A., Liu, S., . Tymokhin. , V.I., Ralph, J., McQueen-Mason, S.J., Bruce, N.C., “. Tsuji Y., . Vanholme. R., . Tobimatsu. Y., Ishikawa Y., Foster C. E., . Kamimura. N., . Hishiyama. S., Hashimoto S., Shino A., Hara H., Sato-Izawa K., . Oyarce. P., . Goeminne. G., . Morreel. K., Kikuchi J., Takano T., Fukuda M., Katayama Y., . Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. Steps in RDT:. depolymerization. Shuai. , . L. . . et al.. . “Formaldehyde stabilization facilitates lignin monomer production during biomass . depolymerization. .. ”. . Science . 354. , 329-333 (. 2016) [. DOI: 10.1126/. Kontur. , W. S. . et al.. . “. Novosphingobium. . aromaticivorans. uses a Nu-class glutathione . S. -transferase as a glutathione . lyase. in breaking the β-aryl ether bond of lignin.” . Journal of Biological Chemistry . 1. S.S. Macdonald, J.H. Pereira, F. Liu, G. Tegl, . A. DeGiovanni, J.F. Wardman, S. Deutsch, Y. Yoshikuni, P.D. Adams, and S.G. Withers, . ACS Cent. Sci.. . 8. , 430 (2022), doi:10.1021/acscentsci.1c01570.