PPT-Unit 1: Introduction to Enzymes Introduction & Definiti

Apoenzymes Holoenzyme Abzymes Synzyme Ribozyme Extremozyme Historical Development in Enzymology Characteristics of enzymes Enzyme Vs Chemical Catalysts Enzyme mechanism amp Activation Energy Enzyme Specificity Fischer and . Definition of an enzyme. Enzyme. is . protein . . catalyst. (i.e. . increase the rate of reactions. ). . NOT. changed during the reaction. Understanding:. Enzymes have an active site to which specific substrates bind. Enzyme catalysis involves molecular motion and the collision of substrates with the active site. Temperature, pH and substrate concentration affect the rate of activity of enzymes. Reactions. Most reactions take place in a number of steps which need to be carefully controlled if the cell is to function properly. Enzymes are the most important controllers of cellular reactions. Catalysts. Enzymes as Catalysts. Enzymes are used to speed up chemical (metabolic) reactions e.g. respiration or photosynthesis- so why use enzymes in industry?. They are specific. can catalyse reactions between specific chemicals, even in a large mixture . Warm up. Grab warm up and complete. We will be putting this into your . journal. When you are done with your warm up look over your Vocab 1-1, you have a . quiz today. Biomolecule review. Cellulose, a structural component of the cell wall of plants and some forms of algae, is mainly used to produce paper and cardboard products. Cellulose would be an example of which type of biomolecule. E S <=> ES <=> ES* <=> EP <=> E P. Michaelis-Menten Kinetics. E S <=> ES <=> ES* <=> EP <=> E P. Rate of Formation. Substrate. Product. Enzyme. Enzyme-. IMPORTANCE. OF ENZYMES. Enzymes are biological catalysts that:. . I. ncrease. . reaction rates . by lowering the amount of energy needed for the reaction to occur. 100-1000x . faster. !!!. Activation Energy. Part 1. . Chapter 3. Learning outcomes. You should be able to:. explain how enzymes work. describe and explain the factors that affect. enzyme activity. use V. max. and K. m. to compare the affinity of different enzymes for their substrates. Food biochemistry and food processing. 2012. Simpson B.K. (Ed.) 2. nd. . edition,. John Wiley & Sons, . Inc.. Enzymes in food processing. Advantages.. Enzymes are proteins with powerful catalytic . Enzyme & Substrate. An enzyme is a globular protein which acts as a biological catalyst by speeding up the rate of chemical reactions. . Enzymes are . NOT. changed or consumed by the reactions they catalyze and thus can be reused. . : An enzymes that breaks down nucleic acid molecule by breaking the phosphodiester bonds at end 3. / . or 5. /.. Endonucleases. : . An Enzyme that hydrolyzes internal phosphodiester bonds in a polynucleotide chain or nucleic acid molecules.. In order for chemical reactions to take place, . enzymes . must be present to help . speed up . the reaction.. Chemical bonds connect atoms. to make . molecules. .. Chemical . reactions. can do two things:. Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. DNA manipulating enzymes:. that . catalyze. (. i.e.. , . increase or decrease the rates. of) . chemical reactions. .. . In enzymatic reactions, the . molecules. at the beginning of the process are called . substrates. , and they are converted into different molecules, called the .