PPT-Identifying Bacteria based on Enzymes and multiple test med

Lab 9 Medgar Evers College Prof Victor Santos Aim To identify bacteria based on the enzymes they have that allow them to carry out certain specific reactions You will detect the presence of . ch. 12. The protein catalyst. Targets:. Describe the relationship between substrate and an active site in enzymatic reactions. Identify the role of coenzymes and enzymatic reactions. List factors that affect enzymatic activity. Essential idea: Enzymes control the metabolism of the cell.. Above is just a small part of the IUBMB-. Sigma-Nicholson Metabolic Pathways Chart . aims to show all the metabolic pathways found in eukaryote cells. The chart in it's entirety shows how complex the chemicals reactions needed to support life in a single cell unit. This was mentioned earlier in 2.1, but in addition to the complexity now add to that . Warm up. Grab warm up and complete. We will be putting this into your . journal. When you are done with your warm up look over your Vocab 1-1, you have a . quiz today. Biomolecule review. Cellulose, a structural component of the cell wall of plants and some forms of algae, is mainly used to produce paper and cardboard products. Cellulose would be an example of which type of biomolecule. Bacteria, friend or foe…?. We have learned lots already about the detriments of bacteria…. They do smell bad…. Disease. Antibiotic resistance. Biofilm formation*. Spoilage. But are these microorganisms vital to our survival?. Enzymes speed up chemical reactions.. Everything that happens in a cell. Growth. Reproduction. Interaction with the environment. Response to stimuli. Is the result of a chemical reaction.. A chemical reaction is a change in the arrangement. Are specific for . what they . catalyze . and do not permanently change during reactions. End in the word -. ase. Function of Enzymes. Enzymes work by lowering the activation energy (the amount of energy needed to start a reaction). . E S <=> ES <=> ES* <=> EP <=> E P. Michaelis-Menten Kinetics. E S <=> ES <=> ES* <=> EP <=> E P. Rate of Formation. Substrate. Product. Enzyme. Enzyme-. IMPORTANCE. OF ENZYMES. Enzymes are biological catalysts that:. . I. ncrease. . reaction rates . by lowering the amount of energy needed for the reaction to occur. 100-1000x . faster. !!!. Activation Energy. 1. What are enzymes?. Each enzyme has only one specific reaction it can perform. . 2. How many different reactions can a single enzyme perform?. Yes, the same enzyme molecule can be used over and over. Enzyme & Substrate. An enzyme is a globular protein which acts as a biological catalyst by speeding up the rate of chemical reactions. . Enzymes are . NOT. changed or consumed by the reactions they catalyze and thus can be reused. . Enzymes. Back when we discussed proteins, we learned that one critical function of protein was to speed up chemical reactions.. Enzymes. are the proteins that increase the rate of chemical reactions. Factors affecting enzymatic reactions. Enzymes specificity. Enzymes kinetics and mechanism of action. Control of metabolic pathways. Enzymes inhibition. Enzymes in clinical diagnosis. Enzymes and genetic diseases. E. coliTotal coliformTotal coliform = Environmental contaminationFecal coliformFecal coliform and E. coli = Fecal contaminationE. coli O157:H7 You should test for coliform bacteria at least once a yea that . catalyze. (. i.e.. , . increase or decrease the rates. of) . chemical reactions. .. . In enzymatic reactions, the . molecules. at the beginning of the process are called . substrates. , and they are converted into different molecules, called the .