PPT-Eukaryotic genomes are complex 3D structures comprised of modified and unmodified DNA,

Most DNA is wrapped around a histone core to form nucleosomes The classical histone protein complexes bind very tightly to DNA and prevent association with other proteins Modifications of the classical histones or their replacement with unusual histone types under certain conditions can loosen the interaction with DNA allowing access to transcription factors RNA polymerase and other proteins . 7. 7 RNA Synthesis and Processing. Chapter Outline. Transcription in Prokaryotes. Eukaryotic RNA Polymerases and General Transcription Factors. Regulation of Transcription in Eukaryotes. RNA Processing and Turnover. & . Genome evolution. Genomes and Genome Evolution - BIOL 4301/6301. What to expect and some suggestions.. I . like think . of . myself as . fair but reasonably tough. I want people to do well but I’m not willing to compromise on the material or ethical guidelines to make it happen.. TSW – Recognize that the presence of a nucleus determines whether a cell is prokaryotic or eukaryotic. 6.12B. Cells. There are two . basic types . of cells. Prokaryotes. or prokaryotic cells . Eukaryotes. TSW – Recognize that the presence of a nucleus determines whether a cell is prokaryotic or eukaryotic. 6.12B. Cells. There are two . basic types . of cells. Prokaryotes. or prokaryotic cells . Eukaryotes. Stryer. Short course. Chapter 33. Nucleic Acid Structure. Nucleobase. Nucleoside. Nucleotide. Nucleic acid. Chromatin. Chromosome. Polymeric Structure. Polymer ideal for informational molecule. Ribose and deoxyribose. Add to Table . of Contents. p. 10 DNA/RNA Vocabulary. p. 11 DNA/RNA Structures. p. 12 Complementary Base-Pairing. DNA. Deoxyribonucleic Acid. Double-stranded. Double-helix structure. Made of nucleotides - each nucleotide has 3 parts (remember PBS). Comparative genomics of sponges, sea anemones, and multicellular pancakes. Mansi Srivastava. Rokhsar Lab, Department of Molecular and Cell Biology, UC Berkeley. Reddien Lab, Whitehead Institute for Biomedical Research. 6.1 Introduction to Genomics. 6.2 Sequencing Genomes. 6.3 Bioinformatics and Annotating Genomes. 6.1 Introduction to Genomics. Genome. Entire complement of genetic information. Includes genes, regulatory sequences, and noncoding DNA. What do all of these organisms have in common?. . They all share a universal genetic code.. DNA and RNA are Nucleic Acids. What is a nucleic acid?. Nucleic acids are one of the major organic biomolecules.. Eukaryotic Cell. 3. Eu. = True. Karyon. = Nucleus. B- The Eukaryotic Cell. Animal Cell. Plant Cell. Compare Animal and Plant cell. What are the functions of cell organelles . ?. Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings. Size of genome. Segmented or not. Site of replication.  . FAMILIES of NEGATIVE STRAND VIRUSES.  . NON-SEGMENTED (-)STRAND VIRUSES. .  . RHABDOVIRIDAE. Rabies, VSV, & Plant viruses .  . FILOVIRIDAE. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. A primary transcript . is the initial, linear, RNA copy of a transcription unit—the segment of DNA between specific initiation and termination sequences. . The primary transcripts of both prokaryotic and eukaryotic . Examples:. Viral transduction into mammalian cells. Agrobacterium transformation of plant cells. Sources: . http://en.wikipedia.org/wiki/Viral_vector. Daniel . Eisenman. , Medical University of South Carolina.