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Volume ml mM final 1 M HEPES pH 79 01 10 1 M MgCl2 0015 15 Volume ml mM final 25 M Sucrose 196 025 1 M MgCl2 02 10 Up to 20 ml 55 035 1 M MgCl2 002 0 Stock Volume

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We also include a simplified version of the protocol, which can be use: Transcript

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