PPT-Determining the Histone Post-translational Modification Binding Specificities of ASH1L

Tiffanie Lee Quantitative Biology Dr Brian Strahl Department of Biochemistry and Biophysics How does ASH1L read chromatin What histone reader domains are involved in binding ASH1L and MLL cooperate to maintain hematopoiesis and transcription in immune cells in the blood. its specificities (partially) determining the nature of its interface and s-selection, k) + C ! RkBy (1), argument structures of predicates provide substantial hints for their structuralrepresentati Y. Lyse & Sonicate. IP. Reverse crosslinks. Total. Reverse crosslinks. Amplify. Amplify. Sequence. Sequence. Immuno. -precipitation. Other controls for IP. (e.g., no antibody, non-specific antibody). Biological Sequence Analysis. BNFO 691/602 Spring . 2014. Mark Reimers. Analysis of ChIP-Seq Data. Genomic Data Analysis Course. Moscow July 2013. Mark Reimers, . Ph.D. What Are the Questions?. Where are histone modifications?. of epigenetics. A. dult. . stem cells know their . fate. !. For example: m. yoblasts. . can . form . muscle . cells. only.. Hematopoetic. . cells only become blood . cells. .. .....b. ut. . all . Pointer. Numerative. Describer/. s. Classifier/. s. Thing. Qualifier/. s. To quantify. . the . thing:. . How many or how much. ?. To point to. . the . thing:. . Which . one. . are you . pointing to?. the sequence specificities of DNA- and RNA-binding proteins is essential for developing models of the regulatory processes in biological systems and for identifying causal disease variants. . -Here . trithorax. -Group (. trxG. ). Maintain the “on” state of developmental regulatory genes. trx. : founding member (HMT on H3K4). . Transcriptional memory by . trxG. and . PcG. trxG. – on state memory. Image: http. ://cliparts.co/clipart/2504918. Determining Latitude. 40°.  . Image: http. ://cliparts.co/clipart/2504918. Determining Latitude. 0. ° . Image: http. ://cliparts.co/clipart/2504918. Determining Latitude. Kiyomi Seiya. PIP-II Collaboration Meeting. 9-10 November 2011. Outline. Resonance circuit . modifications . Injection . energy . stabilization. Cogging . 11/9/2015. Kiyomi Seiya| Booster modification for 20Hz operation. 1. Bioinformatics III. X-ray structure of the nucleosome core particle consisting of core histones, and DNA. Top view.. www.wikipedia.org. Side view shows two windings of DNA and two histone layers. The DNA of eukaryotic organisms is packaged into chromatin, whose basic repeating unit is the . Nucleosome. A packaging unit for DNA (=H3/H4 tetramer + two sets of H2A/H2B . dimer. ). DNA (- charge) and . histones. (+ charge) . histones. = tails and globular domains. Higher-Order Packaging of Chromatins. The UF-FSU hub is supported by the National Center for Advancing Translational Sciences of the National Institutes of Health under University of Florida Clinical and Translational Science Awards UL1TR001427, KL2TR001429 and TL1TR001428.. quantitation. . -. For most K residues our histone assay, we monitor the possible occurrence of difference modifications (. m. e1. , . m. e2. , . m. e3. ,. Ac. ) and . unmodified. peptide. . -Different forms of the same peptide, apart of their mass differences, can have different retention times (Important for . Thimo Rohlf 1,4 , Lydia Steiner 1,2 , Jens Przybilla 1 , Sonja Prohaska 2 , Hans Binder 1,3 and Jörg Galle 1 * 1 Interdisciplinary Centre for Bioinformatics of Leipzig University, D-04107 Leipzig,