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PPT-APPENDIX A SUPPLEMENTARY FIGURES PowerPoint Presentation

A B C D Protospacer Scaffold T7 Promoter sgRNA E Cas9 Fig A1 The synthesis amplification and transcription of the sgRNA and assembly of the RNP A Once designed

cassette hygromycin resistance sgrna hygromycin cassette sgrna resistance region mat127 primers pcr sequence mea fig produce digested length structures

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APPENDIX A SUPPLEMENTARY FIGURES: Transcript

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