PDF-LHoechst33258watersolutionfor5minutes
1
m
Lof10mg100
m
atroomtemperatureinthedarkAfterthisthespermatozoa
suspensionwascentrifugedat340
6
g
for5minutesThepellet
wasresuspendedin1mLofPBSandcentrifugedagainat400
6
g
for5minutesAfterdisc
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