PPT-Phage Strategies Some phages have only a single strategy for survival on infecting a susceptible

In lytic cycle the phage DNA enters the host bacterium its genes are transcribed the phage GM replicated and the protein components of the phage particle are produced Finally the host bacterium is lysed to release the assembled progeny particles by the process of lysis. Mark . Bergland. and Karen . Klyczek. University of Wisconsin-River Falls. . ACUBE Annual Meeting, Lakeland College, . 2012. Case It! Project. URL for . Case It! Home Page:. http. ://caseit.uwrf.edu. Geobacillus. and . Heamophilious. . Phages. Ramey . Elsarrag. Introduction. Tandem repeats are adjacently repeated patterns of DNA sequences. They are associated with genetic instability and are used for forensic identification. LOCK. it out on the first move. HI, DROXYLATE and . SWEET RELEASE defeat METHYLATE. but stay in play until cancelled. If host plays RESTRICT. then phage can counter with. METHYLATE – move both cards to the discard pile. , Nnamdi E. . Ihejirika. , Tamara D. Jones, Lisa R. Mwanza, Prisca C. . Obidike. , Jacqueline G. Ruban, Nathanial J. Sangster,. Anna K. Hull, and David F. Royer . Background and Hypothesis. A phage hunters course was offered at Lincoln University (PA) for the first time during the 2014-15 academic year. The seven students in the course included four freshmen, two sophomores and one senior. Each student successfully isolated a . Series I: . Isolation and Purification of Phage. 1. We created an enrichment culture in order to grow bacteriophages and ultimately isolate a selected phage to examine. . 2. We generated a plaque assay using . Abdulrahman. . Alazemi. , . Shahroze. Abbas, Liam Lewis, Donald Ta, Ann Vo. Overview. Clustered repeats. Wide variety of functions. History largely unknown. Regulatory Sites. A segment capable of altering expression of specific genes. . Josiah Austin, Julia Tang, Tiffany Nguyen, Michael . Ketteh. , Lynn Zhou, Dr. Mao PhD. The M13 phage is a human-friendly bacteriophage that only infects bacteria cells. 1. . It’s a nanofiber-shaped phage with five different coating proteins wrapped around its DNA (Figure 1b). The M13 phage can be engineered to display the exogenous peptides on its surface. This phage display technique allows us to study the function of short peptides that are derived from the cell’s growth factors, or proteins. Additionally, M13 phage have the ability to self-assemble into organized films that look like which can serve as a matrix for a cell culture. The phage film matrix can give us a better understanding of the role of short peptides in growth factors. The traditional method of making phage films requires the use of a pipette to drop the negatively charged phage solution onto the poly-lysine (Figure 2). However, manually, the results are not replicable and the process is time consuming. In order to make more organized and repeatable phage films we use a programmable slide stainer machine (Carl Zeiss, Inc.) that can dip the slides into a each solution allowing time to dry and giving it the ability to agitate. Resultantly, we built phage films while varying soaking time, phage concentration, and the number of phage layers to determine the best conditions to form well-ordered phage films with the help of slide stainer.. . Author: Michael Arnoult, San Diego State University. Mentors: Victor Seguritan, Anca Segall, and Peter Salamon, Department of Biology, San Diego State University. Methods:. Bacteriophages are the single most abundant biological entity on earth, and influence every environment in which bacteria exist. There are no current algorithms which reliably analyze phage structural protein sequences and predict their function. . Mary Owen, Andrew . Schaff. , Kayla Cartwright, Angel Chen, Jessica Clark, Bradley Davis, Paige . Etchison. , Shelby . Frieszell. , Jacquelyn Gross, Steph Smith, Elsa Clenny, . Sammi. . Podolyan. , Stephanie . Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. of . mycobacterial disease. Fernanda . Alonzo, . Adebanjo. . Adedoja. , . Ann . M. Findley, School of Sciences, . ULM; . Chris . R. . Gissendanner. , School of Pharmacy. , . ULM. . Abstract. Nontuberculous . M13. , f1 and . fd. are filamentous . coliphages. . containing a . circular . single-stranded DNA molecule. . These . coliphages. . have . been developed as cloning . vectors, for . they have a . number . HHMI Logistics. . Name badges must be worn at all times. Restrooms. No roaming through the building. Questions? – Ask . us. Refreshments . (keep to the schedule). Technology. Live Streaming. The microphones. or phages are viruses that specifically infect bacteria. . Viral vectors are those in which gene of interest is incorporated in the genome of virus.. The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell..